Titel: Mass Spectrometry-based Phyloproteomics of Clostridioides difficile as alternate Typing Method to Ribotyping is able to differentiate the Ribotype 027
Art: Abstractautor
Session: Workshop 03
Diagnostic Microbiology (StAG DV)

Referent: Matthias F. Emele (Göttingen)

Abstract - Text


Clostridioides difficile, a Gram-positive spore-forming bacterium, is the leading cause of nosocomial diarrhea worldwide and therefore a substantial burden to the healthcare system. Over the past decade, the hypervirulent C. difficile PCR-ribotype (RT) 027 emerged rapidly all over the world what was associated with both, increased severity and mortality rates. 


To prevent disease spread, it is of great importance to identify epidemic strains such as RT027 as fast as possible. Commonly used diagnostic methods, e.g. multi locus sequence typing (MLST) or PCR-ribotyping, are time-consuming, wherefore the aim of the study was to develop a typing scheme based on Mass-spectrometry-based-Phyloproteomics (MSPP), a fast, inexpensive, and reliable method.


To establish a C. coli-MSPP scheme, we analyzed and evaluated 77 ribotyped strains representative for five MLST clades by mass spectrometry. MLST, based on whole genome sequences, and PCR-ribotyping were used as further reference methods.


We were able to associate nine biomarkers with their encoding genes and include them in our C. difficile MSPP scheme. The most important finding was that one of the clades only contained isolates of the hypervirulent RT027.


In this study, the MSPP principle that was previously used for subtyping of several organisms by our group, was now successfully applied to C. difficile. Using this technique, we are able to distinguish RT027 isolates from non-RT027 isolates what makes it fast and valuable alternative to currently used diagnostic tools.