Titel: Development of a specific and sensitive ELISA as an in-vitro diagnostic tool for the detection of Bartonella henselae antibodies in human serum
ID: 21/RKV
Art: Abstractautor
Session: Workshop 04
Molecular Epidemiology of Infectious Diseases (StAG RK, FG MS)

Referent: Volkhard A. J. Kempf (Frankfurt am Main)

Abstract - Text

Question: Bartonella henselae causes cat scratch disease and several other clinical entities. Infections with B. henselae are frequently occurring, however, the infection is only rarely diagnosed mainly due to a lack of knowledge in the medical community. Microscopic immunofluorescence assays (IFA) are widely used for serodiagnostics of B. henselae-infections, but are laborious, time consuming and interpretation is subjective. An easy and reliable method for the serological diagnosis of B. henselae infections is needed to overcome the shortcomings of the current IFA. Here, we report the development of an ELISA detecting human anti-B. henselae antibodies from serum samples.

Methods: By separating the water-insoluble fraction of B. henselae Houston-1 via ion exchange chromatography, 16 subfractions were generated and tested for immunoreactivity via line blotting. One particular fraction (#24) was selected and spotted on ELISA-plates using an industrial production platform.

Results: By the use of well-characterized human sera from the strictly quality-controlled serum library of the German National Consiliary Laboratory for Bartonella infections, sensitivity of this ELISA was 100% for molecular proven infections and 76% for clinically suspected infections at a specificity of 93%, respectively.

Conclusions: This ELISA is therefore a reliable high-throughput method allowing serodiagnostics of B. henselae infections.