Titel: Spectrum of mutations in antibiotics resistance and pathoadaptive loci of Pseudomonas aeruginosa isolates identified with amplicon sequencing
ID: 23/MSV
Art: Abstractautor
Session: Workshop 04
Molecular Epidemiology of Infectious Diseases (StAG RK, FG MS)

Referent: Sebastian Fischer (Hannover)

Abstract - Text

Pseudomonas aeruginosa is an important causative agent of nosocominal infections and is the pathogen which contributes most to the shortened life expectancy in cystic fibrosis (CF) patients. In this project we analyzed mutations of antibiotics resistance and pathoadaptive loci of CF isolates compared to non-CF isolates using fast and cost effective Illumnia amplicon sequencing of 37 loci (17 antibiotics resistance, 20 pathoadaptive loci) in more than 500 isolates to investigate potential differences comparing the habitats. Major differences in the number of amino acid sequence changing variants were found in the investigated loci comparing CF and non-CF isolates ranging from only few amino acid changes (LepA) up to a high number of changes including an accumulation of early stop codons (AlgG, AlgU). Additionally, a correlation of the mutations with the structure of the AlgG and MexZ locus was identified showing evidence for differential functionality in the habitats. These potential function changing isolate shall be tested in vivo to test for function modification.