Structural biologists have made great progress in discovering the structures of individual proteins and protein complexes. Much of the recent progress has resulted from advances in cryo-TEM, especially single-particle analysis, which has made the elucidation of near-atomic-scale structures for small to large proteins and protein complexes almost routine. The ultimate goal is to derive the structures of the molecular machines that constitute all living systems in their natural, functional contexts, where their interactions with other components can be observed. Studying the molecular machinery of cells from atomic detail to the cellular context and beyond is a great challenge for cell biology. This workshop will highlight the latest developments in cryo-electron microscopy. Special attention is given to our advances on productivity enhancements in the context of the Single Particle Analysis (SPA) workflow. Secondly, we will introduce the suite of instruments that enable Thermo Fisher Scientific’s cryo-electron tomography workflow which allow to peer inside cells and see proteins in situ – in their unperturbed functional environments – at nanometer resolution and in 3D.