Session

04.09.2019     12:45–13:45

Title:
Lunchtime lecture
Thermo Fisher Scientific
Type:
Industry session
Room:

This workshop will demonstrate the use of Thermo Scientific™ Amira™ Software with a focus on the segmentation of cryo-electron tomography data. Cryo-electron tomography combines the best structural preservation possible with the power of three-dimensional high-resolution imaging. The method allows to peer inside cells and see proteins in situ – in their unperturbed functional environments. We will introduce the suite of instruments that enable Thermo Fisher Scientific’s cryo-electron tomography workflow and we will demonstrate how data obtained with this workflow can be visualized using Amira Software. The workshop will feature a live demonstration of Amira Software. Based on a real data set, we will demonstrate how the visualization of cryo-tomography data can be facilitated using semi-automated tools for the detection of filamentous cellular structures and image processing routines for enhancing the segmentation of cellular membranes.


04.09.2019
12:45–13:45

Title:
Lunchtime lecture
Thermo Fisher Scientific
Type:
Industry session
Room:

This workshop will demonstrate the use of Thermo Scientific™ Amira™ Software with a focus on the segmentation of cryo-electron tomography data. Cryo-electron tomography combines the best structural preservation possible with the power of three-dimensional high-resolution imaging. The method allows to peer inside cells and see proteins in situ – in their unperturbed functional environments. We will introduce the suite of instruments that enable Thermo Fisher Scientific’s cryo-electron tomography workflow and we will demonstrate how data obtained with this workflow can be visualized using Amira Software. The workshop will feature a live demonstration of Amira Software. Based on a real data set, we will demonstrate how the visualization of cryo-tomography data can be facilitated using semi-automated tools for the detection of filamentous cellular structures and image processing routines for enhancing the segmentation of cellular membranes.



12:45–13:45
Introduction to Amira software for cryo-electron tomography
Jan Giesebrecht (Eindhoven/NL), Alexander Rigort (Eindhoven/NL)
Talk time: 50 + 10 min